Estimation of the Prevalence of Ehrlichia ruminantium in Amblyomma spp. and Blood of Domestic Ruminants in the Sudan by Conventional PCR Based on pCS20 Gene Region
نویسندگان
چکیده
1. Department of Parasitology, Faculty of Veterinary Medicine, University of Al-Butana, Tamboul, Sudan 2. Department of Parasitology, Faculty of Veterinary Medicine,University of Khartoum, Khartoum Sudan 3. Animal Resources Research Corporation Ministry of Animal Resources and Fisheries, Sudan 4. Department of Zoology, Omdurman Islamic University, Faculty of Science, Omdurman, Sudan 5. Department of Medicine and Therapeutic, University of Al-Butana, Faculty of Veterinary Medicine, Tamboul Sudan
منابع مشابه
Ehrlichia ruminantium, Sudan
To the Editor: Ehrlichia ruminantium, the causative agent of heartwater, is transmitted by Amblyomma spp. ticks. Amblyomma variegatum ticks, which are found in the Caribbean and sub-Saharan Africa, except in certain areas of southern Africa, are major vectors of E. ruminantium (1–3). A. lepidum is also an important vector of heartwater, especially in eastern Sudan (4). However, few epidemiologi...
متن کاملA quantitative real-time PCR assay for Ehrlichia ruminantium using pCS20.
Heartwater is a tick borne disease that affects ruminants and wild animals in Africa south of the Sahara. It is caused by Ehrlichia ruminantium and transmitted by the tick Amblyomma hebraeum. The protocols currently used to detect heartwater take several days to complete. Here, we describe the development of a pCS20 quantitative real-time PCR TaqMan probe assay to detect E. ruminantium in lives...
متن کاملDetection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks.
We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR assay and applied it to field ticks. The assay detected DNA of 37 isolates of...
متن کاملSimultaneous detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantium in Amblyomma variegatum ticks by reverse line blot hybridization.
The detection of Anaplasma and Ehrlichia species is usually based on species-specific PCR assays, since no assay is yet available which can detect and identify these species simultaneously. To this end, we developed a reverse line blot (RLB) assay for simultaneous detection and identification of Anaplasma and Ehrlichia species in domestic ruminants and ticks. In a PCR the hypervariable V1 regio...
متن کاملThe pCS20 PCR assay for Ehrlichia ruminantium does not cross-react with the novel deer ehrlichial agent found in white-tailed deer in the United States of America.
White-tailed deer are susceptible to heartwater (Ehrlichia [Cowdria] ruminantium infection) and are likely to suffer high mortality if the disease spreads to the United States. It is vital, therefore, to validate a highly specific and sensitive detection method for E. ruminantium infection that can be reliably used in testing white-tailed deer, which are reservoirs of antigenically or genetical...
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تاریخ انتشار 2014